|
KMID : 1098420100180050305
|
|
Korean Journal of Medicinal Crop Science
2010 Volume.18 No. 5 p.305 ~ p.310
|
|
|
Inhibitory Effect of quercetin-3-O-a-L-rhamnopyranoside from Chamaecyparis obtuse on Aldose Reductase and Sorbitol Accumulation
|
|
Kim Seon-Ha
Kim Jin-Kyu
Lee Yeon-Sil
Bae Young-Soo
Lim Soon-Sung
|
|
|
Abstract
|
|
|
|
Taxifolin-3-O-β-D-xylopyranoside and quercetin-3-O-α-L-rhamnopyranoside were isolated from an EtOAcsoluble extract of the leaves of Chamaecyparis obtuse. Quercetin-3-O-α-L-rhamnopyranoside was found to possess a potent inhibitory activity of human recombinant aldose reductase in vitro, its IC50 value being 11.5 μM. Kinetic analysis showed that quercetin-3-O-α-L-rhamnopyranoside exhibited uncompetitive inhibition against DL-glyceraldehyde. Also, quercetin- 3-O-α-L-rhamnopyranoside suppresses sorbitol accumulation in rat lens under high glucose conditions, demonstrating the potential to prevent sorbitol accumulation in vivo. These results suggest that this compound may be a promising agent in the prevention or treatment of diabetic complications.
|
|
|
KEYWORD
|
|
|
Chamaecyparis obtusa, Taxifolin-3-O-β-D-xylopyranoside, Quercetin-3-O-a-L-rhamnopyranoside, Diabetic Compli-cations, Human Recombinant Aldose Reductase, Sorbitol Accumulation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|